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. 2009 May 22;42(4):541–553. doi: 10.1111/j.1365-2184.2009.00609.x

Figure 7.

Figure 7

Effect of p21 silencing on cells treated with 3‐O‐methylfunicone (OMF). (a–c) RT‐PCR analysis using specific primers for p21, cyclin B1 and p34 mRNA expression. Top: Lane 1, untreated A375P cells mRNA; lane 2, mRNA from p21‐siRNA A375P; lane 3, mRNA from A375P treated with 80 µg/ml OMF for 24 h; lane 4, mRNA from p21‐siRNA A375P treated with 80 µg/ml OMF for 24 h; lane 5, untreated A375M cells mRNA; lane 2, mRNA from p21‐siRNA A375M; lane 3, mRNA from A375M treated with 80 µg/ml OMF for 24 h; lane 4, mRNA from p21‐siRNA A375M treated with 80 µg/ml OMF for 24 h; M, 100‐bp ladder MW marker (Roche Diagnostics). Bottom: Quantitative measurements of band intensities. Data shown are representative of five different experiments (± standard deviation). *Significantly different compared with control (P < 0.05).