Table 1.
Human primer sense and anti‐sense sequences and expected PCR products (base pairs)
| Gene | Sense and anti‐sense sequences | Conditions | Base pairs |
|---|---|---|---|
| p21 | 5′‐CTGCCCAAGCTCTACCTTCC‐3′ | 30 cycles at 94 °C for 60 s | 123 |
| 5′‐CAGGTCCACATGGTCTTCCT‐3′ | 60 °C for 60 s, 72 °C for 60 s | ||
| p27 | 5′‐ATGTCAAACGTGCGAGTGTCTAAC‐3′ | 30 cycles at 95 °C for 60 s | 596 |
| 5′‐TTACGTTTGACGTCTTCTGAGGCCA‐3′ | 63 °C for 60 s, 72 °C for 60 s | ||
| P53 | 5′‐TTCTTGCATTCTGGGACAGCC‐3′ | 30 cycles at 94 °C for 60 s | 650 |
| 5′‐GCCTCATTCAGCTCTCGGAAC‐3′ | 56 °C for 60 s, 72 °C for 60 s | ||
| Cyclin B1 | 5′‐AAGAGCTTTAAACTTTGGTCTGGG‐3′ | 33 cycles at 94 °C for 30 s | 318 |
| 5′‐CTTTGTAAGTCCTTGATTTACCATG‐3′ | 55 °C for 60 s, 72 °C for 60 s | ||
| p34cdc2 | 5′‐GGAGTATAGGCACCATATTTGC‐3′ | 33 cycles at 95 °C for 60 s | 196 |
| 5′‐GACATGGGATGCTAGGCTTCC‐3′ | 54 °C for 60 s, 72 °C for 60 s | ||
| hTERT | 5′‐CGGAAGAGTGTCTGGAGCAA‐3′ | 35 cycles at 95 °C for 45 s | 163 |
| 5′‐GGATGAAGCGGAGTCTGGA‐3′ | 52 °C for 45 s, 72 °C for 45 s | ||
| TEP‐1 | 5′‐TCAAGCCAAACCTGAATCTGAG‐3′ | 31 cycles at 95 °C for 40 s | 264 |
| 5′‐CCCCGAGTGAATCTTTCTACGC‐3′ | 60 °C for 50 s, 72 °C for 50 s | ||
| Survivin | 5′‐ATGAGATACCATGGGTGCCCCGACG‐3′ | 34 cycles at 94 °C for 45 s | 450 |
| 5′‐TTAAGGATCCCTGCTCGATGGCACG‐3′ | 56 °C for 45 s, 72 °C for 60 s | ||
| GAPDH | 5′‐CGGAGTCAACGGATTTGGTCGTAT‐3′ | 306 | |
| 5′‐AGCCTTCTCCATGGTGGTGAAGAC‐3′ | |||
| β‐actin | 5′‐TGACGGGGTCACCCACACTGTGCCCATCTA‐3′ | 661 | |
| 5′‐CTAGAAGCATTGCGGTGGACGATGGAGGG‐3′ |