Table 3.

Effect of p21 siRNA on cell‐cycle distribution and apoptosis of OMF‐treated cells. A375P (untreated A375P), A375M (untreated A375M), siRNA‐OMF1 (p21‐siRNA‐OMF treated A375P), siRNA‐OMF2 (p21‐siRNA‐OMF‐treated A375M)

Groups	Cell cycle
	G0 + G1	S	G2 + M	Sub‐G1
Untreated A375P	63.85 ± 2.89	19.63 ± 2.56	11.65 ± 1.23	0.45 ± 0.28
p21‐siRNA‐OMF untreated A375P	59.70 ± 2.33	21.42 ± 1.85	14.74 ± 3.04	1.30 ± 1.34
p21‐siRNA‐OMF treated A375P	50.65 ± 3.92	18.56 ± 1.68	17.11 ± 1.11	9.46 ± 1.12
p	A375P vs. siRNA‐OMF1 = 0.0046		A375P vs. siRNA‐OMF1 = 0.002	A375P vs. siRNA‐OMF1 = 0.00001
Untreated A375M	62.95 ± 2.94	18.78 ± 3.02	17.65 ± 1.25	0.62 ± 0.16
p21‐siRNA‐OMF untreated A375M	57.27 ± 1.07	19.56 ± 1.58	21.41 ± 1.13	1.09 ± 1.02
p21‐siRNA‐OMF treated A375M	43.05 ± 1.98	21.52 ± 2.34	25.36 ± 2.13	2.34 ± 0.56
p	A375M vs. siRNA‐OMF2 = 0.0003		A375M vs. siRNA‐OMF2 = 0.002	A375M vs. siRNA‐OMF2 = 0.003

Data expressed as mean ± standard deviation of five experiments. Significant P‐values are reported.