Table 1.
PCR primers used in this study for the detection of tissue‐specific marker gene expression
| Gene symbol | Official full name (NCBI reference sequence) | |
|---|---|---|
| Forward (5′→3′) | Reverse (5′→3′) | |
| POU5F1 (Oct3/4) | POU domain, class 5, transcription factor 1 (NM_013633.3) | |
| GGTGGAGGAAGCCGACAAC | TTCGGGCACTTCAGAAACATG | |
| SOX2 | SRY‐box containing gene 2 (NM_011443.3) | |
| AGATGCACAACTCGGAGATCAG | CCGCGGCCGGTATTTATAAT | |
| GATA6 | GATA binding protein 6 (NM_010258.3) | |
| CGGTCATTACCTGTGCAATG | GCATTTCTACGCCATAAGGTA | |
| TTR | transthyretin (NM_013697) | |
| GTCCTCTGATGGTCAAAGTC | TCCAGTTCTACTCTGTACAC | |
| BMP4 | bone morphogenetic protein 4 (NM_007554.2) | |
| CTGCCGTCGCCATTCACTAT | TGGCATGGTTGGTTGAGTTG | |
| NPPA (ANF) | natriuretic peptide type A (NM_008725.2) | |
| CGGTGTCCAACACAGATCTG | TCTCTCAGAGGTGGGTTGAC | |
| NES | nestin (NM_016701.3) | |
| TGCATTTCCTTGGGATACCAG | CTTCAGAAAGGCTGTCACAGGAG | |
| GFAP | glial fibrillary acidic protein (NM_001131020.1) | |
| TGCCACGCTTCTCCTTGTCT | GCTAGCAAAGCGGTCATTGAG | |
| GAPDH | glyceraldehyde‐3‐phosphate dehydrogenase (NM_008084.2) | |
| TGCACCACCAACTGCTTAG | GGATGCAGGGATGATGTTC | |