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. 2015 Mar 27;48(3):284–292. doi: 10.1111/cpr.12176

Figure 2.

Figure 2

Overexpression of miR‐93 promoted type II collagen expression in NP . (a) Expression levels were examined using real‐time PCR for non‐transfected cells or after transfection of 20 nmol/l of miR‐93 mimic or scramble control, after 24 h. (b) Overexpression of miR‐93 promoted type II collagen mRNA expression using real‐time PCR. NP cells were transfected with 20 nmol/l of miR‐93 mimic or scramble control or remained non‐transfected after 24 h. Type II collagen was detected using real‐time PCR. (c) Overexpression of miR‐93 promoted type II collagen protein expression as demonstrated by immunohistochemistry. NP cells were transfected with 20 nmol/l miR‐93 mimic or scramble control or remained non‐transfected after 24 h. Type II collagen was detected by immunohistochemical analysis. Images were acquired using laser scanning confocal microscopy under a 40× objective lens. Values are presented as mean ± SD. ***Indicates P < 0.001.