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. 2015 Mar 27;48(3):284–292. doi: 10.1111/cpr.12176

Figure 4.

Figure 4

Overexpression of MMP3 partially rescued miR‐93‐induced type II collagen expression. (a) Western blot analysis of MMP3 in NP cells co‐transfected with either miR‐93 mimic (20 nm) or scrambled miRNA (20 nm) and either MMP3 vector (2 μg) or empty vector (2 μg). (b) Overexpression of MMP3 reduced type II collagen mRNA expression. NP cells were transfected with MMP3 vector or empty control. Type II collagen expression was detected using real‐time PCR; relative ratio of cells per field is shown. (c) Real‐time PCR assays of type II collagen mRNA expression with NP cells treated as described in (a). (d) Western blotting analyses of type II collagen protein expression of NP cells treated as described in (a). Bars represent mean ± SD. GAPDH was loading control. **Indicates P < 0.01, ***Indicates P < 0.001.