Figure 6.

 Culture of hepatic oval cells obtained from porcine liver. (a) Porcine hepatic oval cells proliferated with number of population doubling at 72 h after plating. (b) Oval cells proliferated faster with increase in number of cells after culture for 6 days. (c) Porcine oval cells retained the ability to proliferate after two passages. Colonies (indicated by arrows) containing tens of cells were seen following a period of oval cell expansion. Scale bars in a–c = 12 μm. (d) Proliferation assay showed number of oval cells after plating (lane 1) and culture for 14 days (lane 2). Note: *P < 0.05.