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Summary of purification of EDDF
| Step | Total protein (mg) | Yield (%) | Purification (fold) |
|---|---|---|---|
| Spleen extract | 83000 | 100 | 1.0 |
| Amonium sulphate | 32000 | 97 | 2.5 |
| IEF(I) | 1200 | 42 | 29 |
| IEF(II) | 65 | 34 | 430 |
| IEF(III) | 12 | 25 | 1700 |
| HPLC (gel filtration) | 4.2 | 16 | 3200 |
| Centricon‐10 | 1.8 | 13 | 5900 |
| RP‐HPLC (c18) | 0.8 | 8.2 | 8500 |
