FIG 6.

Sortin2 acts synergistically with fluconazole against C. glabrata. (A) Broth microdilution assay results for the BG2 wild-type strain in the presence of several concentrations of sortin2 or sortin3 after 48 h of growth at 37°C. Relative OD₆₀₀ values compared to those achieved under the 0-μg/ml fluconazole condition are shown. Dotted lines indicate 50% (upper line) and 90% (lower line) growth inhibition. The MIC₅₀ and MIC₉₀ values can be deduced from the cross-section between the respective dotted lines and the data curves. (B) Tolerance assay results for the BG2 wild-type strain in the presence of several concentrations of sortin2 or sortin3. CFU counts were compared in the absence and the presence of fluconazole (64 μg/ml) relative to those achieved under the 0-μg/ml sortin condition. Statistical analysis was conducted using one-way analysis of variance with the Bonferroni correction. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.