TABLE 1.
Strain | MICFLC (μg/ml) |
||
---|---|---|---|
Etest | Broth microdilution assay |
||
50% | 90% | ||
S. cerevisiae pep3Δ | 2–3 | 4 | 4–8 |
S. cerevisiae pep5Δ | 1–2 | 4 | 4–8 |
S. cerevisiae pep7Δ | 0.75–1.5 | 2 | 2 |
S. cerevisiae pep45Δ | 1.5–3 | 0.5–1 | >128 |
S. cerevisiae pep51Δ | 0.5–0.75 | 4 | 4–8 |
S. cerevisiae pep54Δ | 0.75–1 | 4 | 4–8 |
S. cerevisiae BY4742 | 6–8 | 16–32 | 32 |
C. albicans vps11Δ/Δ | 0.5 | 0.125 | 0.5 |
C. albicans YJB6284 | 0.25 | 0.125 | 0.25 |
C. glabrata vps45Δ/Δ | 8 | 4 | 8 |
C. glabrata ATCC 2001 | 16 | 8 | >128 |
The MICFLC was determined by Etest (after 48 h or 72 h) or broth microdilution assay (after 48 h) of 6 deletion strains of S. cerevisiae (the pep3Δ, pep5Δ, pep7Δ, vps45Δ, vps51Δ, and vps54Δ strains) and the wild-type strain (BY4742), the C. albicans vps11Δ/Δ and C. glabrata vps45Δ/Δ deletion strains, and the respective wild-type strains (YJB6284 and ATCC 2001).