(a) Circular map of pIBAC_IncN plasmid compared with pKp148 (black circular box). Green arrows, conjugal transfer system of the plasmid; red arrow, blaKPC gene; yellow arrows, mobile elements; white arrows, hypothetical proteins; purple arrow, replication protein repN; orange arrow, stability protein stdB. Gray circular box represents the plasmid backbone, and transposon Tn4401i is represented by a blue arrow. (b) Alignment in the position of interest between the sequence of Tn4401i and that of Tn4401b. −35 and −10, putative promoter regions; +1, putative transcription starting point; RBS, ribosome-binding site.