FIG 1.

(A) N. fowleri trophozoites were seeded at 2 × 10⁶ cells/ml in control medium (Nelson’s complete medium) and cultured with or without glucose. (B) Cells (1 × 10⁶ cells/ml) were incubated with or without 3-bromopyruvate (50 mM) for 2 days prior to imaging. Images were captured on an EVOS imaging system, and data are representative of multiple experiments. Scale bar, 20 μM.