FIG 2.

Effects of ATP, glucose, and pH on NfGlck activity. (A) NfGlck (10 nM) activity in response to increasing glucose concentrations (2.44 to 312 μM) (A), increasing ATP concentrations (0.04 to 5.0 mM) (B), and different pH levels (C). Two different buffers with buffering capacity in the range of pH tested were used for the assay, Tris-Cl (pH 7 to 8.5) and sodium carbonate (pH 9.0 to 10.5). For pH analysis, glucose and ATP concentrations were 425 μM and 1.5 mM ATP, respectively. Reactions were carried out in triplicate, and standard deviations are indicated.