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(A) NfGlck (10 nM) activity with various hexoses (5 mM). (B) Impact of phosphoryl-bearing substrates on NfGlck activity. NfGlck activity was measured in the presence of 1.5 mM ATP supplemented with ADP, PPi, and ATP (all at 5 mM). Assays were carried out in triplicate, and standard deviations are indicated.
