TABLE 2.

MICs of the recombined strains^a

Strain	Plasmid	Promoter(s)	MIC (μg/ml)
			IPM	MEM	FEP	CAZ	TZP	ATM
Tn4401A	pTn4401A	P1, P2	64	32	≥256	≥256	≥1,024	≥256
M1Tn4401A	pM1Tn4401A	P1M, P2	32	16	32	128	≥1,024	≥256
M2Tn4401A	pM2Tn4401A	P1, P2M	≤0.5	≤0.5	≤1	≤1	≤4	≤1
Tn4401B	pTn4401B	P1, P2, P3	8	8	32	64	≥1,024	≥256
M1Tn4401B	pM1Tn4401B	P1M, P2, P3	8	8	32	64	≥1,024	≥256
M2Tn4401B	pM2Tn4401B	P1, P2M, P3	≤0.5	≤0.5	≤1	≤1	≤4	≤1
CTB	pCTB	P1, PX	16	16	32	128	≥1,024	≥256
M1CTB	pM1CTB	P1M, PX	4	2	4	16	128	64
MXCTB	pMXCTB	P1, PXM	4	4	4	16	512	128
PXCT	pPXCT	PX	8	8	8	32	512	128
SDKPC	pSDKPC	N	≤0.5	≤0.5	≤1	≤1	≤4	≤1
Trans1-T1			≤0.5	≤0.5	≤1	≤1	≤4	≤1

^aTrans1-T1, competent cell of E. coli DH5α that was used as the recipient strain in this study. IPM, imipenem; MEM, meropenem; TZP, piperacillin-tazobactam; FEP, cefepime; CAZ, ceftazidime; ATM, aztreonam. Superscript M, mutation; N, no bla_KPC promoter available.