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. 2019 Jan 31;52(2):e12568. doi: 10.1111/cpr.12568

Figure 2.

Figure 2

Slc31a1 knock‐down inhibited pancreatic cancer progression. A, after transfection of 50 nmol/L si‐Slc31a1 or NC for 72 h, the mRNA or protein expressions of SLC31A1 in Panc‐1 cells were measured by qPCR or Western blot, respectively. β‐actin was used as an internal control. B and C, the effect of si‐Slc31a1 (50 nmol/L) on Panc‐1 and MiaPaCa‐2 cell growth was measured by CCK‐8 assay for 5 days. D, the migration of Panc‐1 cells transfected with si‐nc or si‐Slc31a1 was tested via wound healing assay. The representative pictures were shown at 0 and 48 h after the wounds were made. E, the invasion of Panc‐1 cells transfected with NC or si‐Slc31a1 was studied via transwell assay. F, the colony formation capacities of Panc‐1 cells transfected with NC or si‐ Slc31a1 were determined. All results are presented as the means ± SD of values obtained in three independent experiments, n = 3, ***P < 0.001 (Student's t test)