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. 2019 Jan 31;52(2):e12568. doi: 10.1111/cpr.12568

Figure 5.

Figure 5

Copper deprivation by si‐Slc31a1 and tetrathiomolybdate (TM) inhibited the mitochondrial activity of pancreatic cancer cells. A, The ROS levels of Panc‐1 and MiaPaCa‐2 cells that were transfected with 50 nmol/L si‐Slc31a1 or treated with 50 μmol/L TM for 24 h were measured by flow cytometry. B, Mitochondrial membrane potential of Panc‐1 transfected with NC or si‐Slc31a1 (50 nmol/L) was tested. Rhodamine123 was used to test mitochondrial membrane potential. C, The GSH/GSSG ratio of Panc‐1 cells that was induced by Slc31a1 interference was presented via GSH/GSSG kit instructions. D, the mitochondrial morphology of Slc31a1 knocked down Panc‐1 cells was presented via transmission electron microscope. The red arrow showed mitochondrial morphology. E, The Slc31a1 knocked down Panc‐1 cells were treated with NAC (1 mmol/L) for 24 h, and ROS levels were then measured via fluorescence detection. F, The ATP levels in Panc‐1 and MiaPaCa‐2 cells were shown after the cells were transfected with si‐Slc31a1 or NC for 48 h. All results are presented as the means ± SD of values obtained in three independent experiments, n = 3, *P < 0.05, **P < 0.01, ***P < 0.001 (Student's t test)