Figure 2.

miR‐297 enhances lung adenocarcinoma cell proliferation and colony formation. (a) The expression of miR‐297 in the lung adenocarcinoma cell lines (H1299, SPC‐A1, H23 and A549) and one bronchial epithelial cell line (16HBE) was measured by qRT‐PCR. (b) The expression of miR‐297 in the A549 cells transfected with miR‐297 mimic was detected using qRT‐PCR. (c) Ectopic expression of miR‐297 enhanced the A549 cells proliferation. (d) Overexpression of miR‐297 promoted the cyclin D1 mRNA expression. (e) The protein expression of cyclin D1 was measured by Western blot. (f) Ectopic expression of miR‐297 promoted cell colony information in the A549 cells. *P<.05, **P<.01 and ***P<.001