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. 2010 Apr 28;43(3):219–228. doi: 10.1111/j.1365-2184.2010.00670.x

Figure 4.

Figure 4

  Effects of Runx2 overexpression on osteoblast/cementoblast‐related gene expressions. Total RNA was isolated from DFCs, NIH3T3 and the cells infected with pLEGFP‐N1, pLEGFP‐IRESRunx2 and pLEGFP‐IRES‐Runx2(M). Real‐time RT‐PCR was performed to investigate the effects of Runx2 overexpression on osteoblast/cementoblast‐related gene expressions, including Col I, OPN, BSP, OC, CAP and CP23. All values were normalized with β‐actin levels and the data were presented as the mean ± SE for three experiments. *P < 0.05 vs. DFCs and DFCs‐EGFP, **P < 0.01 vs. DFCs and DFCs‐EGFP, ††P < 0.01 vs. DFCs‐Runx2. §§P < 0.01 vs. NIH3T3 and NIH3T3‐EGFP, ‡‡P < 0.01 vs. NIH3T3‐Runx2, ‡P < 0.05 vs. NIH3T3‐Runx2. Statistical significance was determined using t‐test.