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. 2009 Dec 27;43(1):1–8. doi: 10.1111/j.1365-2184.2009.00648.x

Figure 5.

Figure 5

 Expression of fast myosin heavy chain altered by hypoxia: RhEpo did not prevent this. L6 myoblasts were cultured for 72 h in growth medium containing 0–10 IU/mL RhEpo at 21% O2 and for 96 h in differentiation medium at 21% or 1% O2. (a) Western blotting shows expression of fast MHC and β‐actin in L6 myoblasts non‐treated (0) or treated, with a range from 1 to 10 IU/mL of RhEpo. (b) Quantification of myosin heavy chain expression was carried out as ratio of myogenin/β‐actin. Western blots are representative of those obtained from three different cultures. Data are presented as mean ± SEM. *P ≤ 0.01, 21% versus 1% O2.