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. 2011 Jun 27;44(4):301–310. doi: 10.1111/j.1365-2184.2011.00765.x

Figure 2.

Figure 2

 Dsg3 silencing in HaCaTs caused reduction in Dsg2 and disruption of desmosome junctions. (a) Confocal images of HaCaT cells transfected with either scrambled siRNA (Scram) or Dsg3 RNAi‐2 and dual labelled for Dsg2 (green, with 33‐3D Ab) and Dsg3 (red, with 5H10 Ab) counterstained with DAPI. Note significant reduction in and disruption of desmosomal junctions (indicated by arrows) was evident in cells with both Dsg3 and Dsg2 depletion. Bar = 10 μm. (b) Hanging drop assay indicated significantly increased particle number and reduced particle size in cells with Dsg3 depletion. Data (mean ± SD) pooled from two independent experiments (sample size = 12).