Figure 4.

 Transiently suppressed cell growth after Dsg3 knockdown. (a) Growth curve of HaCaT cells after transfection with or without scrambled (Scram), RNAi‐1 and RNAi‐2 siRNAs. Note that cells transiently transfected with Dsg3 siRNAs exhibited lower growth rate compared to controls, particularly, RNAi‐2‐treated cells. (b) Cell growth recovery over three passages after transient siRNA transfection in HaCaTs. Cells were seeded at the same density the following day after transfection and allowed to grow for 10 days (P1). Then, all cells were harvested and cell number for each population was determined by direct cell counting before seeding an aliquot of cells for culture again (P2). The same procedure was repeated for P3. Note that population growth rate of cells with Dsg3 knockdown appeared to be recovering gradually over time at P2 and P3.