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. 2007 Sep 14;40(5):685–705. doi: 10.1111/j.1365-2184.2007.00464.x

Figure 7.

Figure 7

Expression of neuronal proteins and receptors in cells exposure of CM or BMP4, then cultured in neuronal differentiation medium. Western blot analysis of nestin, neuronal nuclear protein (NeuN), NMDA receptor 1 (NR1), glutamate receptor 1 (GluR1), tyrosine hydroxylase (TH) and actin. Lane 1 = Control, keratinocytes exposed to 1 : 1 DKSFM : uCM for 24 h, then neuronal differentiation medium for 10 days. Lane 2 = Keratinocytes exposed to 1 : 1 DKSFM : CM for 24 h, then neuronal differentiation medium for 10 days. Lane 3 = Keratinocytes exposed to 1 : 1 DKSFM : uCM +100 ng/mL BMP4, for 24 h, then neuronal differentiation medium for 10 days. Lane 4 = control, keratinocytes grown in 100% DKSFM for 10 days. Lane 5 = P22 W4 ESC grown in ESC medium for 24 h, then neuronal differentiation medium for 10 days. Lane 6 = Skin fibroblasts grown in DMEM for 10 days. 50 µg of protein loaded per lane.