. 2007 Sep 14;40(5):685–705. doi: 10.1111/j.1365-2184.2007.00464.x

Table 2.

Percentage of cells expressing Oct‐4 under various conditions

Treatments	% cells expressing Oct‐4 protein
100% DKSFM	0.0 ^a
1 : 1 DKSFM : uCM	0.0 ^a
1 : 1 DKSFM : ES‐CM	4.2 ± 0.04 ^a
50 ng/mL BMP4	0.0 ^a
100 ng/mL BMP4	4.0 ± 0.30 ^a
50 ng/mL BMP2	3.7 ± 2.11 ^b
100 ng/mL BMP2	4.4 ± 2.08 ^b
50 ng/mL BMP2 +50 ng/mL BMP4	7.9 ± 4.70 ^a
100 ng/mL BMP2 +100 ng/mL BMP4	8.8 ± 5.77 ^a
Keratinocyte : ES cell contact co‐culture	1.6 ± 0.02 ^a
Keratinocyte : ES cell non‐contact co‐culture	4.5 ± 0.04 ^a

means ± SD, ^a n = 4, ^b n = 2. BMP, bone morphogenetic protein; CM, conditioned medium; DKSFM defined keratinocyte‐serum free medium; ES, embryonic stem; Oct‐4, octamer‐4; uCM, unconditioned medium.