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. 2008 Sep 10;41(5):775–785. doi: 10.1111/j.1365-2184.2008.00551.x

Figure 4.

Figure 4

Effects of ClC‐3 knockdown by incubation of 40 nm ClC‐3 siRNA for 48 h, on protein expression of cyclin E, cyclin D1 and cyclin‐dependent kinase inhibitors (CDKIs) p21 and p27. (a) Representive Western blot images of cyclins and CDKIs. (b and c) Densitometric analysis of the effects of ClC‐3 knockdown on expression of cyclin D1 and cyclin E (b), and p21 and p27 (c), respectively. Expression of CDK2 was not changed by ClC‐3 knockdown. (a) control; (b) ET‐1; (c) ET‐1 + LipofectamineTM RNAiMAX; (d) ET‐1 + negative siRNA; (e) ET‐1 + ClC‐3 siRNA (n = 5; †P < 0.05, ‡P < 0.01 versus control; *P < 0.01 versus ET‐1).