Figure 2.
Insulin increases BrdUrd incorporation in Kc cells in a DERK‐dependent manner. Cells were stimulated with insulin 1 µm for 12 h, BrdUrd was added for a further 12 h then incorporation of BrdUrd was determined. Where indicated, cells were also treated with PD98059 (5 mm) or wortmannin (100 nm) for 30 min prior to the addition of insulin. Data are expressed as the mean ± SEM of four individual experiments. Statistical analysis was performed by one‐way analysis of variance and P < 0.05 indicated with respect to control (*) and insulin (#).