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. 2002 Jul 31;35(4):221–235. doi: 10.1046/j.1365-2184.2002.00241.x

Figure 2.

Figure 2

LOOH‐induced CaCo‐2 ODC activity is dependent on cell confluency. Subconfluent (70%) or confluent (7 day) CaCo‐2 cell monolayers were incubated in serum‐free DMEM and exposed to 1 or 5 µM LOOH for 6 h. In parallel experiments, subconfluent or confluent cells grown in serum‐free DMEM were supplemented with 10% fetal bovine serum (FBS) and incubated for 6 h. ODC activity was determined by the liberation of 14CO2. Results are the mean ± SE of four separate experiments performed in triplicate. *P < 0.05 versus untreated control and LOOH‐treated confluent cells.