Figure 1.

mTORC2 affects rearrangement of the F‐actin cytoskeleton in mouse fertilized eggs. (a) The development model of mouse fertilized eggs. G1 phase: 12–21 hours after injecting hCG; S phase: 21–27 hours after injecting hCG; G2 phase: 27–30 hours after injecting hCG; M phase: 30–33 hours after injecting hCG. (b) Staining for F‐actin (red) and DNA (blue) revealed the organization of the F‐actin cytoskeleton in mouse fertilized eggs transfected with RICTOR shRNA (F‐actin: yellow arrow). Scale bar: 20 μm. (c) Western immunoblotting detection of mTORC2 in 200 mouse fertilized eggs treated with shRNA targeted against mTORC2. WB: Western blotting