Figure 6.

The mTORC2/Akt1/Girdin pathway rearranges the F‐actin cytoskeleton in one‐cell stage fertilized eggs. (a) The effect of gene knockdown on the expression of Rictor and the phosphorylation of Girdin‐1417. Extracts of mouse fertilized eggs were resolved by 6% SDS‐PAGE, transferred to nitrocellulose and probed with phosphor‐Girdin‐Ser1417 antibody and Girdin antibody. (b) We examined Girdin phosphorylation in the fertilized eggs treated with Rictor shRNA, WT‐Akt1 mRNA, Rictor shRNA and WT‐Akt1 coinjection. In WT‐Akt1 mRNA treated cells, we noted that Girdin was highly phosphorylated at 1417. But cells treated with Rictor siRNA and WT‐Akt1 exhibited decreased phosphorylation of Girdin 1417