Effects of NRAGE knockdown on odontoblastic differentiation of mDPCs. Effect of NRAGE knockdown on mRNA level of odontoblastic marker genes in mDPCs on days 0, 7 and 14: (a) DMP1, (b) DSPP and (c) ALP. GAPDH was used as normalization control. (d) ALP activity in mDPC/shNRG group after 0, 7 and 14 days induction. Protein levels of DMP1 and DSP by western blot analysis on days 0, 7 and 14. *Significant difference (P < 0.05) versus control, **Significant difference (P < 0.01) versus control.