Table 2.
Predictions of resistant mutations on the basis of FV value calculations
| Residue no. | Experimental
K /K
|
FVdouble value | Resistant? Experimental* (Prediction†) | Prediction accuracy | ||||
|---|---|---|---|---|---|---|---|---|
| Saquinavir | ||||||||
| V32 | 1.6–7.3 (V32I) (26) | −0.4 ± 0.3 | N (N) | 75% | ||||
| M46 | 1.0 (M46I) (27) | −0.8 ± 0.2 | N (N) | |||||
| I47 | 1.0 (I47V) (27) | +1.9 ± 0.6 | N (N) | |||||
| G48 | 13.5 (G48V)(28) 163.6 (G48V) (25) | +2.5 ± 1.2 | Y (N) | |||||
| I50 | 21.0 (I50V) (27) | −0.2 ± 0.6 | Y (N) | |||||
| V82 | 0.7–3.7 (V82A/F/I) (26) 3.3–7.3 (V82F/A/I) (29) | +1.1 ± 0.5 | N (N) | |||||
| I84 | 5.8 (I84V) (26) 10.7 (I84V) (29) 12.0 (I84V) (27) | −0.8 ± 0.5 | N (N) | |||||
| L90 | 3.0 (L90M) (28, 30) 20.7 (L90M) (25) | −0.4 ± 0.0 | N (N) | |||||
| Amprenavir | ||||||||
| M46 | 1.0 (M46I) (27) | −1.7 ± 0.2 | N (Y) | 86% | ||||
| I47 | 1.0 (I47V) (27) | +1.5 ± 0.6 | N (N) | |||||
| G48 | 3.5 (G48V) (25) | +3.7 ± 1.1 | N (N) | |||||
| I50 | 83.0 (I50V) (27) | −2.1 ± 0.5 | Y (Y) | |||||
| V82 | 0.4–3.3 (V82A/F/I) (29) | +0.9 ± 0.6 | N (N) | |||||
| I84 | 23.0 (I84V) (27) 2.7 (I84V) (29) | −1.2 ± 0.5 | Y (Y) | |||||
| L90 | 2.7 (L90M) (25) | −0.7 ± 0.0 | N (N) | |||||
| Ritonavir | ||||||||
| M46 | 4.0 (M46I) (27) | −0.5 ± 0.2 | N (N) | 57% | ||||
| I47 | 3.0 (I47V) (27) | +1.5 ± 0.7 | N (N) | |||||
| G48 | 66.7 (G48V) (25) | +5.0 ± 1.0 | Y (N) | |||||
| I50 | 10.0 (I50V) (27) | −0.2 ± 0.5 | N (N) | |||||
| V82 | 0.8–14.7 (V82A/F/I) (29) | +0.7 ± 0.5 | Y (N) | |||||
| I84 | 11.2 (I84V) (29) 20.0 (I84V) (27) | −0.0 ± 0.5 | Y (N) | |||||
| L90 | 6.7 (L90M) (25) | −0.3 ± 0.0 | N (N) | |||||
| Indinavir | ||||||||
| V32 | 8.0 (V32I) (26) | −0.9 ± 0.3 | N (N) | 86% | ||||
| M46 | 4.3 (M46I) (26) | −1.6 ± 0.2 | N (Y) | |||||
| I47 | 3.0 (I47V) (27) | +1.0 ± 0.7 | N (N) | |||||
| G48 | 6.3 (G48V) (25) | +3.4 ± 1.1 | N (N) | |||||
| V82 | 0.6–6.4 (V82A/F/I) (29) 6.9–84.7 (V82A/F/I) (26) | +0.2 ± 0.5 | N (N) | |||||
| I84 | 2.6 (I84V) (29) 10.0 (I84V) (26) | −0.9 ± 0.6 | N (N) | |||||
| L90 | 5.8 (L90M) (25) | −0.7 ± 0.0 | N (N) | |||||
| Nelfinavir | ||||||||
| G48 | 1.0 (G48V) (25) | +4.0 ± 1.0 | N (N) | 75% | ||||
| V82 | 0.8–17.5 (V82F/A/I) (29) | +1.5 ± 0.5 | N (N) | |||||
| I84 | 3.5 (I84V) (29) | −1.2 ± 0.5 | N (Y) | |||||
| L90 | 3.5 (L90M) (25) | −0.7 ± 0.0 | N (N) | |||||
Average prediction accuracy of five drugs is 76%. N, no; Y, yes.
*
If
K
/K
> 10 for single mutation, that residue is considered to be resistant.
†
The criterion used for predicting resistance is FVdouble ≤ −1.0 (in parentheses).