Figure 3.

Combined treatment of the inhibitors of CXCR4 (AMD3100), PI3K (LY294002) or Akt (SH‐5), partly antagonized the effective of SDF‐1 on cardiomyogenesis. (a) Representative RT‐PCR gene expression profiles of murine BMDCs supplemented with SDF‐1 or mixture of SDF‐1 and different inhibitors. In the presence of any inhibitor, atrial natriuretic factor (ANF) and β‐MHC were obviously down‐regulated. (b–d) Quantification of density ratio of mRNA encoding ANF (b), β‐MHC (c) and connexin 43 (d) to glyceraldehyde‐3‐phosphate dehydrogenase (GAPD). After treatment with each inhibitor, a trend towards down‐regulation of ANF or β‐MHC mRNA was observed, even though the difference was not statistically significant, whereas no obvious change was found in connexin 43 mRNA. Five samples were tested for the target mRNA, and three RT‐PCRs were separately performed for each sample. Mean density ratio of target mRNA to GAPD in each sample was used for statistical analysis.