Figure 5.

Phosphorylation of Akt was measured by the ratio of phospho‐Akt to total Akt. The phospho‐Akt/Akt ratio was lowered when BMDCs were cultured in medium supplemented with SDF‐1 and LY294002, a specific PI3K inhibitor, or SH‐5, a specific Akt inhibitor. Four samples were tested for phospho‐Akt and total Akt, and three Western blots were separately performed for each sample. Mean density ratio in each sample was used for statistical analysis.