Table 1.

Primers for reverse transcription‐polymerase chain reaction

Product (bp)	Forward primer	Reverse primer
Murine ANF (282 bp)	5′‐TTGGCTTCCAGGCCATATTG‐3′	5′‐AAGAGGGCAGATCTATCGGA‐3′
Murine connexin 43 (243 bp)	5′‐ACTTCAGCCTCCAAGGAGTTC‐3′	5′‐GTGTTACAGCGAAAGGCAGAC‐3′
Murine β‐MHC (203 bp)	5′‐GCCAACACCAACCTGTCCAAGTTC‐3′	5′‐TGCAAAGGCTCCAGGTCTGAGGGC‐3′
Murine NKX2.5 (217 bp)	5′‐CAGTGGAGCTGGACAAAGCC‐3′	5′‐TAGCGACGGTTCTGGAACCA‐3′
Murine GAPD (156 bp)	5′‐TTCCAGTATGACTCCACTCACG‐3′	5′‐AGACTCCACGACATACTCAGCA‐3′
Human ANF (456 bp)	5′‐ATGAGCTCCTTCTCCACCAC‐3′	5′‐TCAGTACCGGAAGCTGTTAC‐3′
Human connexin 43 (232 bp)	5′‐AGGCGTGAGGAAAGTACCAA‐3′	5′‐ACACCTTCCCTCCAGCAGTT‐3′
Human β‐MHC (335 bp)	5′‐ATCAAGGAGCTCACCTACCAG‐3′	5′‐AGCTGTTACACAGGCTCCAG‐3′
Human NKX2.5 (205 bp)	5′‐GAGAGTTTGTGGCGGCGATT‐3′	5′‐CGACGGCGAGATAGCAAAGG‐3′
Human β‐actin (587 bp)	5′‐CCAAGGCCAACCGCGAGAAGATGAC‐3′	5′‐AGGGTACATGGTGGTGCCGCCAGAC‐3′

ANF, atrial natriuretic factor; GAPD, glyceraldehyde‐3‐phosphate dehydrogenase; MHC, myosin heavy chain.