Table 1.

 Anti‐tumour effects of 1 and 2 against B16 in C57BL/6J mice

Treatment	Dosage (mg/kg/day) ×day	Mice (n) (initial/end)	Body weight (g) (initial/end)	Tumour weight (g)	Inhibition rate (%)
Normal saline		8/8	18.26/21.07	1.84 ± 0.70
1	0.25 × 21	8/8	18.79/19.68	0.56 ± 0.22*	69.8
1	0.50 × 21	8/8	18.41/18.32	0.24 ± 0.12*	87.2
2	0.10 × 21	8/8	18.32/18.87	0.80 ± 0.36**	56.7
2	0.40 × 21	8/8	18.53/18.40	0.32 ± 0.17*	82.5

B16 cell suspensions were injected subcutaneously into the right axilla region of C57BL/6J female mice (18 ± 2 g), and daily intraperitoneal injections of normal saline or test samples commenced 1 day after injection of tumour cells. All mice were killed on day 21 and the tumours were dissected out and weighed. Data are expressed as mean ± standard deviation.

*P < 0.001 versus normal saline group. **P < 0.005 versus normal saline group.