Skip to main content
. 2003 Jan 28;36(1):35–44. doi: 10.1046/j.1365-2184.2003.00252.x

Figure 2.

Figure 2

Expression of ( truncated) human Bcl‐2 protein in transfected rat CC531 cells. The expression of human Bcl‐2 on western blot is determined using the mAb #6C8: in parental CC531 cells and CC531 cells transfected with the empty vector (CCneo cells), full length bcl‐2 (CCbcl2 cells) or truncated bcl‐2 gene constructs, missing the coding sequence for the BH1 (CCΔBH1 cells), BH3 (CCΔBH3 cells), BH4 (CCΔBH4 cells), or the TM domain (CCΔTM cells). Additionally, we include CC531 cells transfected with the human bax gene (CCbax cells). The 22 kDa immunoreactivity in CCbcl2 cells could reflect caspase‐(like)‐mediated cleavage of the Bcl‐2 protein at Aspartate residue 34 (Asp34), releasing the BH4 domain. Note that this band appears at similar height on a nitrocellulose filter as the Bcl‐2 protein missing the BH4 domain in CCΔBH4 cells. Immunoreactivity of 19 kDa in CCbcl2, CCΔBH3 (profoundly), and to lesser extent in CCΔTM cells, might result from caspase‐(like) cleavage at Asp64.