Table 1.
Cell cycle distribution (%) and GSH levels (nmol GSH/mg total cell protein) of 50 and 100% confluent parental CC531 cell lines , neo‐transfected CC531 cells, and CC531 cells transfected with full‐length bcl‐2 or truncated bcl‐2 gene constructs. a Denotes statistically significant differences in cell cycle distribution at 50 or 100% confluence compared with parental CC531 cells and neo‐transfected cells. Data represent mean ± SD. n = 4–10 for flow cytometric analyses on 50% confluent cell lines, n = 3 for 100% confluent cell populations with all samples in duplicates. a Denotes statistically significant differences in total GSH levels at either 50 or 100% confluence compared with parental CC531 cells and neo‐transfected cells. b Denotes statistically significant differences in GSH levels within one cell line at 50 or 100% confluence. n = 4–6 with samples in duplicates. Data represent mean ± SD
| 50% confluence | 100% confluence | |||||||
|---|---|---|---|---|---|---|---|---|
| Cell cycle | GSH | Cell cycle | GSH | |||||
| G0/G1 (%) | S (%) | G2/M (%) | G0/G1 (%) | S (%) | G2/M (%) | |||
| CC531 | 15.6 ± 3.7 | 63.0 ± 2.7 | 21.4 ± 1.6 | 21.9 ± 2.1 | 54.1 ± 1.0 | 35.1 ± 0.9 | 10.8 ± 0.4 | 30.1 ± 2.3 b |
| CCneo | 17.3 ± 4.2 | 61.7 ± 3.9 | 21.0 ± 2.2 | 22.9 ± 0.8 | 54.3 ± 0.6 | 35.2 ± 0.6 | 10.5 ± 0.3 | 29.6 ± 2.7 b |
| CCbcl2 | 35.5 ± 1.4 a | 46.1 ± 1.3 a | 18.4 ± 0.9 | 30.9 ± 2.0 a | 63.7 ± 1.4 | 23.9 ± 0.3 | 12.4 ± 1.0 | 30.0 ± 2.3 |
| CCΔBH1 | 26.8 ± 2.9 a | 51.1 ± 1.4 a | 22.1 ± 1.5 | 28.4 ± 4.0 a | 51.9 ± 0.5 | 38.0 ± 0.4 | 10.1 ± 0.2 | 31.7 ± 4.0 |
| CCΔBH3 | 25.5 ± 2.4 a | 51.5 ± 3.4 a | 23.0 ± 1.3 | 32.8 ± 2.2 a | 56.4 ± 0.4 | 31.3 ± 5.0 | 12.3 ± 1.4 | 28.2 ± 1.4 b |
| CCΔBH4 | 19.2 ± 2.7 | 60.9 ± 0.9 | 19.9 ± 1.3 | 48.5 ± 7.9 a | 59.8 ± 0.5 | 33.4 ± 2.0 | 6.8 ± 1.2 | 41.9 ± 1.9 a |
| CCΔTM | 29.3 ± 5.7 a | 49.1 ± 3.9 a | 21.6 ± 2.8 | 37.0 ± 4.1 a | 61.2 ± 1.4 | 31.5 ± 0.7 | 7.3 ± 0.3 | 27.8 ± 2.3 b |