Cell cycle distribution (%) and GSH levels (nmol GSH/mg total cell protein) of 50 and 100% confluent parental CC531 cell lines
, neo‐transfected CC531 cells, and CC531 cells transfected with full‐length bcl‐2 or truncated bcl‐2 gene constructs.
a
Denotes statistically significant differences in cell cycle distribution at 50 or 100% confluence compared with parental CC531 cells and neo‐transfected cells. Data represent mean ± SD.
n
= 4–10 for flow cytometric analyses on 50% confluent cell lines,
n
= 3 for 100% confluent cell populations with all samples in duplicates.
a
Denotes statistically significant differences in total GSH levels at either 50 or 100% confluence compared with parental CC531 cells and neo‐transfected cells.
b
Denotes statistically significant differences in GSH levels within one cell line at 50 or 100% confluence.
n = 4–6 with samples in duplicates. Data represent mean ± SD