Figure 3.
Effect of Cu on alkaline phosphatase ( ALP ) expression and matrix mineralization of osteogenic differentiated rat bone marrow mesenchymal stem cells ( rBMSC s). rBMSCs from the third passage under a bright field microscope (a). Scale bars represent 500 μm, original magnification: ×40. General view of Alizarin red S staining (b) rBMSCs differentiation as induced by an osteogenic differentiation inducing medium supplemented with 5 μmol/l CuSO4 for 7 days. Scale bars represent 1 cm. Active ALP of the control group (c), Cu group (d), Osteo group (e) and Osteo+Cu group (f) were detected. Scale bars represent 200 μm, original magnification: ×100. After 21 days of culture, photos were taken under an inverted phase‐contrast microscope for the control group (g), Cu group (h), Osteo group (i) and Osteo+Cu group (j). Scale bars represent 100 μm, original magnification: ×200. Matrix mineralization of the control group (k), Cu group (l), Osteo group (m) and Osteo+Cu group (n) were visualized by Alizarin red S staining. Scale bars represent 200 μm, original magnification: ×100.