Figure 3.

Pre‐treatment with rapamycin enhances migration of UC‐MSCs by upregulating expression of CXCR4. A, Relative mRNA expression of CXCR4 was determined by real‐time polymerase chain reaction. B, Expression of CXCR4 protein was detected by Western blotting assays. Results of statistical analysis of relative density of CXCR4 are shown. C, Representative images of migration of UC‐MSCs in a scratch migration assay after addition of rapamycin, rapamycin +CXCL12 or rapamycin +AMD3100 (×100). Scale bar: 200 µm. Results of statistical analysis of the number of migrated cells are shown. Data are presented as the mean ± standard error of the mean. D, Representative images of migration of UC‐MSCs in a Transwell system after addition of rapamycin, rapamycin +CXCL12 or rapamycin +AMD3100. Scale bar: 200 µm. Results of statistical analysis of the number of migrated cells are shown. Data are presented as the mean ± standard error of the mean. *P < 0.05, **P < 0.01, ***P < 0.001 (all one‐way analysis of variance). UC‐MSCs, umbilical cord‐derived mesenchymal stem cells