Figure 2.

Decrease in radiation‐induced VEGF secretions by roscovitine in (a) MDA‐MB 231 cells and (b) HMEC‐1 cells. The content of VEGF was measured by ELISA on cultured supernatants from exponential growing cells during the indicated incubation time point. (c) Reduction of HMEC‐1 cells invasion by the roscovitine + IR‐conditioned medium of MDA‐MB 231 cells. Invasion of HMEC‐1 endothelial cell was measured by Matrigel invasion chamber assay. Data represent the mean of at least three independent experiments performed in triplicate; error bars, SD. *P < 0.05; Student's t‐test statistical analysis.