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. 2014 Jan 31;47(2):161–171. doi: 10.1111/cpr.12091

Figure 2.

Figure 2

Acrolein ( Acr ) caused lower ratio of GSH / GSSG and induced dose‐dependent ROS generation. GC‐1 cells were exposed to various concentrations of Acr for 6 h, stained with DTNB to measure intracellular GSH and the ratio of GSH/GSSG by Microplate Reader (a and b); or exposed to various concentrations Acr for 4, 6 and 8 h, stained with DCFH‐DA to measure intracellular level of ROS by flow cytometry (c). GSH and the ratio of GSH/GSSG were reduced when they were treated with Acr. Acr caused higher intracellular ROS levels in MGCs. Results showed that ROS increased with concentration and disposal time of Acr. *P < 0.05, compared to 0 μm. **P < 0.05, compared to 0 μm. #P < 0.05, compared to 0 μm 4 h. ##P < 0.05, compared to 0 μm 6 h. †P < 0.05, compared to 0 μm 8 h.