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Inability of acrolein ( Acr ) to alter MGC proliferation. Cells were treated with 0, 0.5, 10 and 100 μm concentrations Acr for 12 h, stained with PI to analyse the cell cycle (a) and treated for 48 h, then stained with CFDA SE to analyse cell generation (b). Both cell cycle and generation analysis above proved that proliferation of the cells was not the reason for cell viability reduction, treated by Acr.
