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Rapamycin relieved acrolein ( Acr )‐induced apoptosis. To confirm the result above, GC‐1 cells were exposed to various concentrations of Acr for 36 h, and stained with propidium iodide (PI) to analyse DNA content by flow cytometry (a and b). Acr induced apoptosis in a concentration‐dependent manner. However, rapamycin relieved this phenomenon. *P < 0.05, compared to 10 μm Arc. #P < 0.05, compared to 100 μm Acr.
