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. 2014 Jan 31;47(2):161–171. doi: 10.1111/cpr.12091

Figure 8.

Figure 8

Rapamycin ( Rap ) alleviated acrolein ( Acr )‐driven intracellular ROS production. GC‐1 cells exposure to Acr and Rap for 6 h. To measure intracellular level of ROS by flow cytometry, cells were stained with DHE (a) or DCFH‐DA (b). The two experiments proved that Acr induced intracellular ROS in a dose‐dependent manner, and Rap‐alleviated Acr caused higher intracellular ROS levels. *P < 0.05, compared to 0 μm Arc. **P < 0.05, compared to 0.5 μm Acr. #P < 0.05, compared to 10 μm Acr. ##P < 0.05, compared to 100 μm Acr.