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. 2014 Jan 31;47(2):161–171. doi: 10.1111/cpr.12091

Figure 9.

Figure 9

Rapamycin ( Rap ) increased Bcl2/Bax ratio in acrolein ( Acr )‐treated cells. GC‐1 cells exposure to Acr and 0.1 μm Rap for 6 h. Bcl2 and Bax mRNA levels were examined with QRT‐PCR (a) the control value was set as 1; protein levels were examined using western blotting (b). The results were analysed by ImageJ (V1.48d) for their integrated density (c). We found that Rap specifically alleviated Acr‐related increase in Bcl2/Bax expression rate. (a) *P < 0.05, compared to 0 μm Arc in Bcl2. **P < 0.05, compared to 0 μm Acr in Bax. #P < 0.05, compared to 0 μm Acr in Bcl2/Bax. ##P < 0.05, compared to 100 μm Acr in Bax. †P < 0.05, compared to 100 μm Acr in Bcl2/Bax. (c) *P < 0.05, compared to 0 μm Arc in Bax. **P < 0.05, compared to 100 μm Acr in Bax. #P < 0.05, compared to 0 μm Acr in Bcl2/Bax. ##P < 0.05, compared to 100 μm Acr in Bcl2/Bax.