Figure 2.

Two‐dimensional pattern of [³⁵S]methionine‐prelabelled proteins extracted from BAE cells. BAE cells were seeded and 24 h later, their proteins were prelabelled with [³⁵S]methionine (3.7 MBq/ml). They were incubated without (a) or with TNF‐α (30 ng/ml) in the presence of CHX (2 µg/ml) (b) for 6h. The cells were then lysed and their proteins were submitted to 2D‐PAGE as described in Materials and methods. V, vimentin; v, high MW degradation products of vimentin (‘staircase’); A, actin; open arrowhead, spots of reduced intensity in treated cells; closed arrowhead, spots observed in treated cells but not in control cells. Spots observed on one pattern gel but not on the other one were localized on the latter by an open circle to facilitate the analysis of the patterns.