Skip to main content
. 2006 Mar 15;39(2):93–103. doi: 10.1111/j.1365-2184.2006.00373.x

Figure 1.

Figure 1

Transcription of the TIIMPSC gene is specifically enhanced by IFN‐α stimulation. (a) mRNA differential display of gene responses induced by IFN‐α. The mRNA was harvested from 5×106 KMB‐17 cells untreated/treated with 100 µ/ml IFN‐α over time. Lane 1, negative control, untreated cells; lanes 2, 3 and 4, mRNA extracted from KMB‐17 cells treated with IFN‐α for 2, 4 and 6 h, separately. Arrowed differential band indicates the TIIMPSC transcript. (b) Nucleotide sequence and deduced amino acid sequence of TIIMPSC. (c) Northern blotting of TIIMPSC expression in human embryo fibroblasts stimulated with IFN‐α. KMB‐17 cells mock‐treated or treated with IFN‐α, and total mRNA isolated for northern blotting. The TIIMPSC transcript was detected using a specific probe (a fragment of 77 nt, nucleotides 1‐77 of TIIMPSC). β‐Actin mRNA was detected as a control with a 491‐nucleotide probe. Lane 1, KMB‐17 cells control; lane 2, KMB‐17 cells treated with IFN‐α for 6 hours.