Table 1.

 Initial cell density and available surface area for the control plate and microcarrier culture conditions

Culture type	Fed	CG/ml	Initial cell density		Medium volume (ml)	Available area (cm2)
			104 cells/ml	104 cells/cm2
PC	–	–	10.66	2.22	2	9.6
			5.33	1.11
			3.22	0.67
MC	–	3.0	10.00	2.22	50	∼225
			5.00	1.11
		5.0	5.00	0.67	50	∼375
MC Bead‐T	0	3.0	5.00	1.11	50	∼225
	1		7.35 ± 1.89	1.63 ± 0.42	100	∼450
	2		7.20 ± 0.82	1.60 ± 0.18	200	∼900

CG, CultiSpher‐G; MC, microcarrier culture; PC, plate culture.