Table 2.

 Primers used for RT‐PCR

Gene name	Primer sequence	Product (bp)
β‐actin	F: 5′‐CGCCAACCGCGAGAAGAT‐3′	168
	R: 5′‐CGTCACCGGAGTCCATCA‐3′
ALP	F: 5′‐TGGAGCTTCAGAAGCTCAACACCA‐3′	454
	R: 5′‐ATCTCGTTGTCTGAGTACCAGTCC‐3′
cbfa1	F: 5′‐CTCACTACCACACCTACCTG‐3′	320
	R: 5′‐TCAATATGGTCGCCAAACAGATTC‐3′
OPN	F: 5′‐CATCTCAGAAGCAGAATCTCC‐3′	313
	R: 5′‐CCATAAACCACACTATCACCTC‐3′
PTH‐R1	F: 5′‐CACAGCCTCATCTTCATGG‐3′	357
	R: 5′‐GCATCTCATAGTGCATCTGG‐3′
C/EBPα	F: 5′‐GTCGGTGGACAAGAACAGC‐3′	234
	R: 5′‐ATGGCCTTGACCAAGGAGC‐3′
PPARγ2	F: 5′‐GCTGTTATGGGTGAAACTCTG‐3′	351
	R: 5′‐ATAAGGTGGAGATGCAGGCTC‐3′

ALP, alkaline phosphatase; C/EBPα, CAAT/enhancer‐binding protein α; cbfa1, core binding factor alpha 1; F, forward; OPN, osteopontin; PPARγ2, proliferator‐activated receptor γ2; PTH‐R1, parathyroid hormone receptor type 1; R, reverse.