Table 3.

 Growth kinetics and metabolic parameters obtained from PCs and MCs at day 6 and MC Bead‐T at day 9

Culture type	Fed	Initial cell density		Maximal cell density		μavg a (h−1)	μmax b (h−1)	Y Lac/Glc c	Fold increase
		104 cells/ml	104 cells/cm2	104 cells/ml	104 cells/cm2
PC	–	10.66	2.22	25.77 ± 0.29	5.16 ± 0.59	0.047 ± 0.001	0.025 ± 0.009	1.04 ± 0.03	2.42
		5.33	1.11	25.44 ± 0.29	5.09 ± 0.59	0.038 ± 0.001	0.027 ± 0.002	1.18 ± 0.04	4.77
		3.22	0.67	25.59 ± 2.23	5.33 ± 0.47	0.020 ± 0.001	0.036 ± 0.003	1.32 ± 0.05	7.99
MC	–	10.00	2.22	26.90 ± 3.61	5.98 ± 0.80	0.012 ± 0.002	0.034 ± 0.001	0.97 ± 0.06	2.84
		5.00	1.11	24.20 ± 0.94	5.38 ± 0.21	0.016 ± 0.001	0.033 ± 0.009	1.02 ± 0.05	4.85
		5.00	0.67	34.70 ± 3.16	4.63 ± 0.42	0.019 ± 0.004	0.041 ± 0.001	1.14 ± 0.16	6.94
MC Bead‐T	0	5.00	1.11	14.70 ± 3.78	3.27 ± 0.84	0.022 ± 0.004	0.043 ± 0.014	0.68 ± 0.02	2.95
	1	7.35 ± 1.89	1.63 ± 0.42	14.40 ± 1.65	3.20 ± 0.37	0.013 ± 0.005	0.028 ± 0.013	0.80 ± 0.03	5.79
	2	7.20 ± 0.82	1.60 ± 0.18	13.90 ± 0.79	3.09 ± 0.17	0.015 ± 0.003	0.023 ± 0.002	1.49 ± 0.12	11.18

CG, CultiSpher‐G; MC, microcarrier culture; PC, plate culture.

^aAverage growth rate (μ_avg) represents the number of doublings per unit time before the death phase.

^bMaximal growth rate (μ_max) represents the growth rate during the log phase.

^c Y _Lac/Glc indicates the yield of lactate from glucose using the following equation: .